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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.45 No.4 pp.158-165

Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus

In-Ohk Ouh1,3#, Ju-Yeon Lee1#, Seo Young Moon1, Jae Young Song1, Sang Ho Jang2, Kwang Myun Cheong2, Choi-Kyu Park3†, Yoon-Hee Lee1†
1Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk 39660, Republic of Korea
2Mediandignostic Inc., Chuncheon 200-883, Republic of Korea
3College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Republic of Korea


A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.