ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.45 No.4 pp.158-165
DOI : https://doi.org/10.13041/jpvm.2021.45.4.158
DOI : https://doi.org/10.13041/jpvm.2021.45.4.158
Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus
Abstract
A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.