Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.44 No.4 pp.201-206
DOI : https://doi.org/10.13041/jpvm.2020.44.4.201

Cytotoxicity evaluation of different commercially available absorbent feminine hygiene products and a transdermal patch using direct contact and extract exposure methods

Alisha Wehdnesday Bernardo Reyes,Tran Xuan Ngoc Huy,Son Hai Vu,Trang Thi Nguyen,Wongi Min,Hu Jang Lee,Suk Kim
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University;Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

Abstract

We investigated the cytotoxic potential of three different commercially available absorbent feminine hygiene products and one transdermal patch using direct contact and extract exposure methods. Two different cell lines were used – mouse fibroblast L929 and normal human skin fibroblast CCD-986sk cell lines. The test samples were extracted using three different methods in accordance to International Organization for Standardization (ISO). Viability of cells was analyzed using MTT assay and morphology of the cells were also observed using phase contrast microscopy. Overall, the direct contact method using L929 cells showed that all the test samples had no toxic effect when exposed to extracts for 1 h. For the exposure method, no toxic effect was observed in both L929 and CCD986sk cells incubated with all the test samples regardless of the extraction methods used.

초록

Figure

Table