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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.44 No.2 pp.96-98
DOI : https://doi.org/10.13041/jpvm.2020.44.2.96

Production of VP2 protein of canine parvovirus-2c expressed in baculovirus expression system

Seyeon Park#,Ju-Yeon Lee#,Jae Young Song,Yoon-Hee Lee,Hyeonhae Choi,Seo Young Moon,Min Ji Kim,Suyeong Yun,Soo-dong Cho,Jienny Lee,Bang-hun Hyun,In-ohk Ouh
Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbjk-do, Republic of Korea
# These authors equally contributed.
Corresponding Author. In-ohk Ouh, Tel: +82-54-912-0802, Fax: +82-54-912-0812, E-mail: dvmoio@korea.kr

Abstract

Viral protein 2 (VP2), which is the structural protein of parvovirus, can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. VP2 of canine parvovirus (CPV) is responsible for neutralizing antibodies in immunized animals. In this study, VP2 protein of canine parvovirus-2c was expressed using a baculovirus expression system and assembled into parvovirus-like particles in insect cells. The results show that VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and a high hemagglutination (HA) titer (1:27). The recombinant 6-His-tagged VP2 protein with a molecular mass of about 65 kDa was detected by anti-His antibody and anti-PPV serum. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of CPV and in the vaccination against CPV.

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