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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.44 No.1 pp.1-4
DOI : https://doi.org/10.13041/jpvm.2020.44.1.1

Quantitative assessment of the food-borne Salmonella contamination using a quantitative real-time polymerase chain reaction

Yewon Cheong1,Ji Seung Jung1,Won Jae Lee2,Jun Bong Lee1,Jang Won Yoon1†
1College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
2College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
Corresponding Author. Jang Won Yoon, Tel: +82-33-250-8791, Fax: +82-33-259-5625, E-mail: jwy706@kangwon.ac.kr

Abstract

Salmonella is one of the most important bacterial pathogens responsible for many zoonotic food-related infectious diseases. Quantitative detection of the foodborne Salmonella contamination in various food sources is therefore critical for preventing the related disease outbreaks. In this study, we developed and evaluated a reliable real-time polymerase chain reaction (RT-PCR) assay to detect the Salmonella contamination quantitatively. The experimental results showed that our invA gene-specific quantitative RT-PCR (qRT-PCR) assay provides a strong correlation between the Cq values and the direct plate counts of Salmonella species in the artificially formulated samples. Further study may be necessary to identify more accurate correlation and equation that can apply to Salmonella spp.

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