Journal Search Engine
Search Advanced Search Adode Reader(link)
Download PDF Export Citaion korean bibliography PMC previewer
ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.43 No.4 pp.239-241
DOI : https://doi.org/10.13041/jpvm.2019.43.4.239

High-yield production of porcine parvovirus VP2 protein of Korean strain assembled into virus-like particles in a baculovirus expression system

Seyeon Park1#,Ju-Yeon Lee1#,Jae Young Song1,Soo-dong Cho1,Jienny Lee1,Bang-hun Hyun1,In-Ohk Ouh1,2†
1Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongsangbuk-do, Republic of Korea
2College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu 41566, Republic of Korea
# These authors equally contributed.
Corresponding Author. In-Ohk Ouh, Tel: +82-54-912-0812, Fax: +82-54-912-0812, E-mail: dvmoio@korea.kr

Abstract

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

초록

Figure

Table