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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.43 No.3 pp.79-87

Seroconversion, seroprotection and safety determination of formalinised local isolate of Aeromonas hydrophila administered through different routes in African catfish (Clarias gariepinus, Burchell 1822)

Saka Babatunde Akeem1†,Adeyemo Olanike Kudirat1,Odeniyi Olubusola Ayoola2,Alarape Selim Adewale1,Odeseye Adebola Olayemi3
1 Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Nigeria
2 Department of Microbiology, University of Ibadan, Nigeria
3 Nigerian Institute of Science Laboratory Technology Ibadan, Nigeria
Corresponding Author. Saka Babatunde Akeem, Tel: +2348030791028,


Motile aeromonad septicaemia (MAS) mostly caused by Aeromonas hydrophila is a disease of high economic impact in freshwater organism. This study examined the seroconversion and seroprotection of formalinised local isolates of Aeromonas hydrophila in the Clarias gariepinus administered via different routes. Highly virulent Aeromonas hydrophila was treated with 40v/v formalin for 24 h. Bacterin was suspended to 0.5 McFarland standard and utilized to immunize Clarias gariepinus of 400±20 g in three groups with 50 samples each via three routes of administration. Antibody measurement taken at regular intervals before and after vaccination revealed a significantly higher antibody values in all routes compared to the control and difference was noticed at all intervals. Relative percent survival was 71.43, 62.86 and 57.14 intraperitoneal, intramuscular and immersion routes, respectively. Correlation analysis established a strong and direct relationship in the antibody generation through route of administration and efficacy of vaccine. This local vaccine was able to confer a high degree of immunity on Clarias gariepinus and can therefore be used to vaccinate against MAS.