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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.43 No.2 pp.74-77

Metacercariocidal effects and infection status of Gymnophalloides seoi in oysters

Hyeon Cheol Kim1#,Eui Ju Hong2#,Si Yun Ryu2,Joon Seok Chae3,Jinho Park4,Kyoung Seong Choi5,Do Hyeon Yu6,Bae Keun Park2†
1College of Veterinary Medicine, Kangwon National University, Chuncheon 24289, Republic of Korea
2College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea
3Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea
4College of Veterinary Medicine, Chonbuk National University, Cheongju 28644, Republic of Korea
5College of Ecology and Environmental Science, Kyungpook National University, Sangju 37224, Republic of Korea
6College of Veterinary Medicine, Gyeongsang National University,Jinju 52828, Republic of Korea
# These authors equally contributed.
Corresponding Author. Bae Keun Park, Tel: +82-42-821-6785, Fax: +82-42-821-8903,


We studied the infection rate of and various metacercariocidal approaches to controlling Gymnophalloides seoi for prevention of human infection in cultured and natural oysters in Korea. The selected survey areas were Aphae-do (Shinan-gun, Jeollanam-do), which is an endemic area for G. seoi, and Tongyeong (Geonsangnam-do), which is the main production area of oysters in Korea. In the Tongyeong area, the metacercariae of G. seoi were not detected in cultured oysters (0/201) or wild oysters (0/134). Seventy-two G. seoi metacercariae were observed in 33 of 265 natural oysters collected from Aphae-do; however, metacercariae were not detected in the cultured oysters (0/1101) purchased from the Daejeon Fish Market. To investigate the viability of G. seoi metacercariae, various metacercariocidal treatments were used with 3.5% saline and oyster juice used as positive controls. The metacercariae survived for 75.4 h in 3.5% saline and 112.6 h in oyster juice. After the metacercariocidal treatment, G. seoi metacercariae were survived for 13.29 min in tap water, < 20 sec in 4.3% vinegar, no effect in a rinse of the whole oyster body in 70°C water for 1 sec, but 1 sec in a rinse of the whole oyster body in 90°C water for 1 sec. The greatest metacercariocidal effect on G. seoi was from rinsing oysters in 90°C water followed by those from treatment with 20% ethyl alcohol, 4.3% vinegar, and tap water. However, we suggest that the most actual prevention to G. seoi human infection is rinsing the oysters with tap water for at least 30 min.