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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.43 No.1 pp.26-30

Evaluation of the adjuvants effect on the immunoglobulin class switching in generation of monoclonal antibodies against Paenibacillus larvae

Jeong-Ah Yu1,Suyeon Kim1,Eun-Ju Ko2,Hong-Gu Joo1,Jaehyuk Yang3,Yoon-Kyu Lim1†
1College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea
2Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA
3Department of equine industry, Korea National College of Agriculture and Fisheries, Jeonju 54874, Republic of Korea
Corresponding Author. Yoon-Kyu Lim, Tel: +82-64-754-3367, Fax: +82-64-756-3354


American foulbrood (AFB) is caused by the bacterium Paenibacillus larvae, which is highly contagious and often lethal to honeybee broods. To control AFB, rapid diagnostic tools including those based on immunological methods are required. We produced several specific mouse monoclonal antibodies (MAbs) against P. larvae. Interestingly, a few of the MAbs were revealed to be an IgM-type antibody. To ascertain the effects of adjuvants on immunoglobulin isotype switching, BALB/c mice were immunized with various adjuvants, i.e., Freund's adjuvant (FA), Alum adjuvant, and AddaVax™ followed by the generation of hybridoma that secreted monoclonal antibodies to P. larvae. In the case of AddaVax™, all screened hybridoma clones secreted IgG-type MAbs, whereas hybridomas generated by Alum and FA secreted 91.25% (7/80) and 66.67% (11/33) respectively, IgG-type MAbs. Although the mechanism of incomplete immunoglobulin isotype switching associated with the P. larvae antigen needs further study, our results indicate that the applied adjuvants can have a significant effect on immunoglobulin isotype switching results.