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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.43 No.1 pp.19-25
DOI : https://doi.org/10.13041/jpvm.2019.43.1.19

Comparison of protective efficacy of Stx2eA toxoid with Salmonella ghosts carrying F18+STEC antigenic proteins against lethal challenge in the murine model

Gayeon Won,Minsoo Choi,John Hwa Lee
College of Veterinary Medicine, Chonbuk National University, Iksan campus, Gobong-ro 79 Iksan, 54596, Republic of Korea
Corresponding Author. John Hwa Lee, Tel: +82-63-850-0940, Fax: +82-63-850-0910

Abstract

Shiga toxin 2e (Stx2e) has a pivotal role in the colonization and enterotoxicity of F18+Shiga toxin-producing Escherichia coli (STEC), which causes porcine edema disease (ED). In this study, a Stx2eA mutant, which has a Glu167Gln mutation in Stx2eA that inactivates N-glycosidase activity, was genetically engineered to evaluate its potential immunogenicity and protective efficacy. A significant increase in serum IgG1 (a Th2 indicator) was shown in mice immunized with the mutated Stx2eA. However, only 56% of the mice immunized with the toxoid (5 μg) survived following a challenge with a lethal dose 50 (LD50) of a virulent F18+STEC strain (JOL654), while mice immunized with Salmonella ghosts delivering selected antigens of F18+STEC showed an 86% survival rate. The results suggest that sole use of the mutated Stx2eA toxoid may not be an effective preventive strategy for the control of porcine ED.

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