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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.42 No.4 pp.171-176

Determination of florfenicol residues in swine tissues using high-performance liquid chromatography with ultraviolet photometric detector

Won-Seok Chae1,Chang-Yeul Yoo2,Lütfiye Tutkun3,Suk Kim4,Hu-Jang Lee4†
1Schools of Life-Chemistry, Daejin University, Pocheon 11159, Republic of Korea
2Department of Computer Software Science, Gyeongnam Provincial Namhae College, Namhae 52422, Republic of Korea
33Department of Medical Biochemistry, Bozok University, Yozgat 66900, Turkey
4Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju 52828, Republic of Korea
Corresponding Author. Hu-Jang Lee, Tel: +82-55-772-2352, Fax: +82-55-772-2308,


A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient (R2) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and 0.040 μg/mL, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.