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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.42 No.4 pp.133-142

Analysis of high-fat diet-induced inflammatory responses in Rhbdf2 knockout mice

Sung-Jun Kim1,Ki-Hoan Nam2,Seul-Gi Park1,Young-Sub Byun2,Eun-Kyoung Kim2,Sang-Mi Cho2,Ha-rim-Kim2,Hyoung-Chin Kim2,Hu-Jang Lee3†,Beom Jun Lee1†
1College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
2Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology, Chungbuk 28116, Republic of Korea
3College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Chinju 52828, Republic of Korea
Corresponding Authors.
Beom Jun Lee, Tel: +82-55-772-2352, Fax: +82-55-772-2308, E-mail:
Hu-Jang Lee, Tel: +82-55-772-2352, Fax: +82-55-772-2308, E-mail:


This study investigated the characteristics of obesity induced by a high-fat diet (HD) over 13 weeks in Rhbdf2 gene knockout (KO) mice. Forty 7-week-old Rhbdf2 wild and KO mice were used and the mice were divided into 4 groups: Wild-ND (n=10, Rhbdf2 wild mice, normal diet (ND)), Wild-HD (n=10, Rhbdf2 wild mice, HD), KO-ND (n=10, Rhbdf2 KO mice, ND) and KO-HD (n=10, Rhbdf2 KO mice, HD). The relative epididymal fat weight in KO-HD was significantly increased compared with that in KO-ND (P<0.01). The relative liver and spleen weights in KO-HD were decreased compared with those in Wild-HD (p < 0.05) and KO-ND (p < 0.01). The mRNA expression of SOD1 in KO-ND was significantly reduced compared with that in Wild-ND (p < 0.05). In Wild-ND and HD, the mRNA expressions of TNF-α and IL-6 in epididymal fat were significantly increased compared with those in KO-ND and HD (p < 0.01). A significant increase of TNF- α and IL-6 mRNA expression was observed in KO-HD compared with KO-ND (p < 0.01). These results indicated that Rhbdf2 genes may regulate high fat diet-induced obesity damage by anti-inflammatory and anti-oxidative roles in fat tissue of mice.