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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.42 No.2 pp.80-85

Naturally produced honey by feeding chitosan to the honeybees

Geon Kim1,Youngmin Yun2,Young Heun Jee2,Chen-Gum Oh3,ByoungSu Yoon4,Jaehyuk Yang5,Yoon-Kyu Lim2†
1Major of Plant Resources Science and Environment, College of Agriculture & Life Sciences, SARI, Jeju National University, Jeju 63243, Republic of Korea
2Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea
3Kunpoong Bio, Seoul 07573, Republic of Korea
4Department of Life Science, Kyonggi University, Suwon 16227, Republic of Korea
5Department of Equine Industry, Korea National College of Agriculture and Fisheries, Jeonju 54874, Republic of Korea
Corresponding Author. Yoon-Kyu Lim, Tel: +82-64-754-3367, Fax: +82-64-756-3354, E-mail:


Chitosan is attracting attention as a health supplement material because of its various physiological activities. In this study, sugar solution containing chitosan was fed to honey bees to induce the production of ‘chitosan fortified honey’ by their same mode of natural honey production. To accomplish this, sugar solutions containing 0.1%, 1%, 2% and 5% chitosan were fed to the honey bees. Fully inverted Chitosan-honey was harvested after feeding the chitosan in sugar solution. To investigate the anti-obesity and immune-enhancing effects of Chitosan-honey, 1% and 10% Chitosan-honey containing drinking water were administrated freely to C57BL mice. Glucosamine concentrations in serum rapidly increased to peak levels in 10 minutes (1261.0 ± 97.6 ng/ml), then decreased gradually for more than 24 hours 793.0 ± 34.7 ng/ml. There were no significant differences in weight and or splenocyte proliferative capacity among experimental mice groups. However, increased granulocytes and monocytes were observed upon flow cytometric analysis. These results suggest that Chitosan-honey could induce removal of foreign antigens. In conclusion, ‘Chitosan-honey’ developed in this study has the potential for use as a honey type dietary health supplement with the same bioactivity as chitosan; however additional research should be conducted to confirm these effects.