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ISSN : 2287-7991(Print)
ISSN : 2287-8009(Online)
Journal of the Preventive Veterinary Medicine Vol.40 No.1 pp.12-21

Canine mesenchymal stem cells immunomodulate atopic dermatitis through the induction of regulatory T cells in an ex vivo experimental study

Jeong Su Byeon1#, Jienny Lee1#, Do Hyung Kim2, Gyeong-Been Lee1, Hee-Ryang Kim1, Na-Yeon Gu1, In-Soo Cho1, Sang-Ho Cha1†
1Viral Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-Ro, Gimcheon-Si, Gyeongsangbuk-Do 39660, Republic of Korea
2KNOTUS, 189 Donggureung-Ro, Guri-Si, Gyeonggi-Do, Republic of Korea
Corresponding Author. Sang-Ho Cha, Tel: +82-54-912-0805, Fax: +82-54-912-0812,


Mesenchymal stem cells (MSCs) are multipotent stem cells, which can be induced to differentiate into several cells. MSCs are also reported to possess immunomodulatory properties through secretion of inflammatory cytokines and generation of regulatory T (Treg) cells. Treg cells play an important role in allergic disorders, including atopic dermatitis. We examined the immunomodulatory effects of canine adipose tissue derived-MSCs (cAD-MSCs) in 3 groups: Group 1, untreated normal dog; Group 2, dogs with Dermatophagoides farinae ointment-induced atopic dermatitis; and Group 3, dogs with atopic dermatitis. Canine peripheral blood mononuclear cells (PBMCs) were collected from each group and co-cultured with cAD-MSCs. After co-culturing, PBMCs were separated and the expression of Treg cells was examined by flow cytometry. According to the results, the populations of Treg cells were increased in 3 ex vivo experimental groups, co-cultured with cAD-MSCs. These results would be important for the application of MSCs in clinical trials.